Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healing

Liu，Qiqi1; Zhang，Ying1,2; Huang，Jingkai3; Xu，Zhourui1; Li，Xiang1; Yang，Jingyu1; Huang，Haoqiang1; Tang，Shiqi1; Chai，Yujuan1; Lin，Jinbo2; Yang，Chengbin1; Liu，Jia2; Lin，Suxia4

2022-12-01

10.1186/s12951-022-01600-9

Journal of Nanobiotechnology   影响因子和分区

1477-3155

The colonization of bacterial pathogens is a major concern in wound infection and becoming a public health issue. Herein, a core–shell structured Ag@MSN (silver core embedded with mesoporous silica, AM)-based nanoplatform was elaborately fabricated to co-load ciprofloxacin (CFL) and tumor necrosis factor-α (TNF-α) small interfering RNA (siTNF-α) (AMPC@siTNF-α) for treating the bacterial-infected wound. The growth of bacterial pathogens was mostly inhibited by released silver ions (Ag) and CFL from AMPC@siTNF-α. Meanwhile, the loaded siTNF-α was internalized by macrophage cells, which silenced the expression of TNF-α (a pro-inflammatory cytokine) in macrophage cells and accelerated the wound healing process by reducing inflammation response. In the in vivo wound model, the Escherichia coli (E. coli)-infected wound in mice almost completely disappeared after treatment with AMPC@siTNF-α, and no suppuration symptom was observed during the course of the treatment. Importantly, this nanoplatform had negligible side effects both in vitro and in vivo. Taken together, this study strongly demonstrates the promising potential of AMPC@siTNF-α as a synergistic therapeutic agent for clinical wound infections. Graphical Abstract: [Figure not available: see fulltext.].

Antibacterial Mesoporous silica siRNA Skin infection Synergistic therapy Wound healing

SCI ; EI

英语

其他

Natural Science Foundation of Guangdong Province[2019A1515012163];National Natural Science Foundation of China[81900264];Science, Technology and Innovation Commission of Shenzhen Municipality[JCYJ20210324130609027];

WOS:000843549600002

20223512641190

Antibiotics ; Cell death ; Macrophages ; Mammals ; Mesoporous materials ; Metal ions ; Silica ; Silver nanoparticles

Medicine and Pharmacology:461.6 ; Biology:461.9 ; Immunology:461.9.1 ; Metallurgy:531.1 ; Nanotechnology:761

BIOLOGY & BIOCHEMISTRY

2-s2.0-85136366411

Scopus

1.Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging,School of Biomedical Engineering,Health Science Center,Shenzhen University,Shenzhen,518060,China
2.Central Laboratory,The Second Affiliated Hospital,School of Medicine,Longgang District People’s Hospital of Shenzhen,The Chinese University of Hong Kong,Shenzhen,518172,China
3.Dermatology Department,Southern University of Science and Technology Hospital (SUSTech Hospital),Shenzhen,518055,China
4.Center of Assisted Reproduction and Embryology,The University of Hong Kong-Shenzhen Hospital,Shenzhen,518048,China

Liu，Qiqi,Zhang，Ying,Huang，Jingkai,et al. Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healing[J]. Journal of Nanobiotechnology,2022,20(1).

Liu，Qiqi.,Zhang，Ying.,Huang，Jingkai.,Xu，Zhourui.,Li，Xiang.,...&Lin，Suxia.(2022).Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healing.Journal of Nanobiotechnology,20(1).

Liu，Qiqi,et al."Mesoporous silica-coated silver nanoparticles as ciprofloxacin/siRNA carriers for accelerated infected wound healing".Journal of Nanobiotechnology 20.1(2022).