Sensitively detecting antigen of SARS-CoV-2 by NIR-II fluorescent nanoparticles

Hu, Ruibin1; Liao, Tao2; Ren, Yan3; Liu, Wenming2; Ma, Rui1; Wang, Xinyuan1; Lin, Qihui3; Wang, Guoxin2; Liang, Yongye1

2022-05-01

10.1007/s12274-022-4351-1

Nano Research   影响因子和分区

1998-0124

1998-0000

Early detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is an efficient way to prevent the spread of coronavirus disease 2019 (COVID-19). Detecting SARS-CoV-2 antigen can be rapid and convenient, but it is still challenging to develop highly sensitive methods for effective diagnosis. Herein, a lateral flow assay (LFA) based on fluorescent nanoparticles emitting in the second near-infrared (NIR-II) window is developed for sensitive detection of SARS-CoV-2 antigen. Benefiting from the NIR-II fluorescence with high penetration and low autofluorescence, such NIR-II based LFA allows enhanced signal-to-background ratio, and the limit of detection is down to 0.01 ng.mL(-1) of SARS-CoV-2 antigen. In the clinical swab sample tests, the NIR-II LFA outperforms the colloidal gold LFA with higher overall percent agreement with the polymerase chain reaction test. The clinical samples with low antigen concentrations (similar to 0.015-similar to 0.068 ng.mL(-1)) can be successfully detected by the NIR-II LFA, but fail for the colloidal gold LFA. The NIR-II LFA can provide a promising platform for highly sensitive, rapid, and cost-effective method for early diagnosis and mass screening of SARS-CoV-2 infection.

coronavirus disease 2019 (COVID-19) severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen detection the second near-infrared (NIR-II) fluorophores lateral flow assay

SCI ; EI

英语

第一 ; 通讯

Guangdong Provincial Department of Science and Technology-key research and development project[2020B1111160003] ; Shenzhen Science and Technology Innovation Commission technology breakthrough project[JSGG20191231141403880] ; Shenzhen San-Ming Project[SZSM201809085] ; Shenzhen Science and Technology Innovation Commission general project[JCYJ20180504165657443]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied

WOS:000793002800007

TSINGHUA UNIV PRESS

20222012102939

Antigens ; Cost effectiveness ; Diagnosis ; Fluorescence ; Gold nanoparticles ; Infrared devices ; Polymerase chain reaction

Medicine and Pharmacology:461.6 ; Immunology:461.9.1 ; Light/Optics:741.1 ; Nanotechnology:761 ; Biochemistry:801.2 ; Industrial Economics:911.2

Web of Science

1.Southern Univ Sci & Technol China, Dept Mat Sci & Engn, Shenzhen 518055, Peoples R China
2.WWHS Biotech Inc, Shenzhen 518122, Peoples R China
3.Longhua Ctr Dis Control & Prevent, Joint Lab Infect Dis Prevent & Control, Hyg Sect, Shenzhen 518109, Peoples R China

Hu, Ruibin,Liao, Tao,Ren, Yan,et al. Sensitively detecting antigen of SARS-CoV-2 by NIR-II fluorescent nanoparticles[J]. Nano Research,2022,15(8):7313-7319.

Hu, Ruibin.,Liao, Tao.,Ren, Yan.,Liu, Wenming.,Ma, Rui.,...&Liang, Yongye.(2022).Sensitively detecting antigen of SARS-CoV-2 by NIR-II fluorescent nanoparticles.Nano Research,15(8),7313-7319.

Hu, Ruibin,et al."Sensitively detecting antigen of SARS-CoV-2 by NIR-II fluorescent nanoparticles".Nano Research 15.8(2022):7313-7319.