Distinct Urinary Metabolic Biomarkers of Human Colorectal Cancer

Zhu, Chang1,2; Huang, Fengjie3; Li, Yang1,2; Zhu, Chaowei1,2; Zhou, Kejun3; Xie, Haihui2,4; Xia, Ligang1,2; Xie, Guoxiang3

2022-04-26

10.1155/2022/1758113

DISEASE MARKERS   影响因子和分区

0278-0240

1875-8630

Colorectal cancer (CRC) is one of the most commonly diagnosed cancers with high mortality rate due to its poor diagnosis in the early stage. Here, we report a urinary metabolomic study on a cohort of CRC patients (n =67) and healthy controls (n =21) using ultraperformance liquid chromatography triple quadrupole mass spectrometry. Pathway analysis showed that a series of pathways that belong to amino acid metabolism, carbohydrate metabolism, and lipid metabolism were dysregulated, for instance the glycine, serine and threonine metabolism, alanine, aspartate and glutamate metabolism, glyoxylate and dicarboxylate metabolism, glycolysis, and TCA cycle. A total of 48 differential metabolites were identified in CRC compared to controls. A panel of 12 biomarkers composed of chenodeoxycholic acid, vanillic acid, adenosine monophosphate, glycolic acid, histidine, azelaic acid, hydroxypropionic acid, glycine, 3,4-dihydroxymandelic acid, 4-hydroxybenzoic acid, oxoglutaric acid, and homocitrulline were identified by Random Forest (RF), Support Vector Machine (SVM), and Boruta analysis classification model and validated by Gradient Boosting (GB), Logistic Regression (LR), and Random Forest diagnostic model, which were able to discriminate CRC subjects from healthy controls. These urinary metabolic biomarkers provided a novel and promising molecular approach for the early diagnosis of CRC.

SCI

英语

通讯

Key Projects of Basic Research in Shenzhen[JCYJ20210324120206017] ; Guangdong Medical Science and Technology Research Foundation Project[B2020052] ; Shenzhen Key Medical Discipline Construction Fund[SZXK015]

Biotechnology & Applied Microbiology ; Genetics & Heredity ; Research & Experimental Medicine ; Pathology

Biotechnology & Applied Microbiology ; Genetics & Heredity ; Medicine, Research & Experimental ; Pathology

WOS:000795097600002

HINDAWI LTD

CLINICAL MEDICINE

Web of Science

1.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Dept Gastrointestinal Surg, Shenzhen 518020, Guangdong, Peoples R China
2.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen 518020, Guangdong, Peoples R China
3.Human Metabol Inst Inc, Shenzhen 518109, Guangdong, Peoples R China
4.Jinan Univ, Clin Med Coll 2, Shenzhen Peoples Hosp, Dept Gynaecol, Shenzhen 518020, Guangdong, Peoples R China

Zhu, Chang,Huang, Fengjie,Li, Yang,et al. Distinct Urinary Metabolic Biomarkers of Human Colorectal Cancer[J]. DISEASE MARKERS,2022,2022.

Zhu, Chang.,Huang, Fengjie.,Li, Yang.,Zhu, Chaowei.,Zhou, Kejun.,...&Xie, Guoxiang.(2022).Distinct Urinary Metabolic Biomarkers of Human Colorectal Cancer.DISEASE MARKERS,2022.

Zhu, Chang,et al."Distinct Urinary Metabolic Biomarkers of Human Colorectal Cancer".DISEASE MARKERS 2022(2022).