3D NIR-II Molecular Imaging Distinguishes Targeted Organs with High-Performance NIR-II Bioconjugates

Zhu, Shoujun1; Herraiz, Sonia2,3; Yue, Jingying1; Zhang, Mingxi1; Wan, Hao1,4; Yang, Qinglai4,5; Ma, Zhuoran1; Wang, Yan2; He, Jiahuan2; Antaris, Alexander L.1; Zhong, Yeteng1; Diao, Shuo1; Feng, Yi2; Zhou, Ying1; Yu, Kuai1; Hong, Guosong1; Liang, Yongye4; Hsueh, Aaron J.2; Dai, Hongjie1

2018-03-27

10.1002/adma.201705799

ADVANCED MATERIALS   影响因子和分区

0935-9648

1521-4095

Greatly reduced scattering in the second near-infrared (N I R - 11) region (1000 1700 nm) opens up many new exciting avenues of bioimaging research, yet N I R-11 fluorescence imaging is mostly implemented by using nontargeted fluorophores or wide-field imaging setups, limiting the signalto-background ratio and imaging penetration depth due to poor specific binding and out-of-focus signals. A newly developed high-performance NIR-II bioconjugate enables targeted imaging of a specific organ in the living body with high quality. Combined with a home-built NIR-II confocal set-up, the enhanced imaging technique allows 900 gm-deep 3D organ imaging without tissue clearing techniques. Bioconjugation of two hormones to nonoverlapping NIR-II fluorophores facilitates two-color imaging of different receptors, demonstrating unprecedented multicolor live molecular imaging across the NIR-II window. This deep tissue imaging of specific receptors in live animals allows development of noninvasive molecular imaging of multifarious models of normal and neoplastic organs in vivo, beyond the traditional visible to NIR-I range. The developed NIR-II fluorescence microscopy will become a powerful imaging technique for deep tissue imaging without any physical sectioning or clearing treatment of the tissue.

3D N IR-II molecular imaging high-performance NIR-II bioconjugate live ovary imaging multicolor live molecular imaging NIR-II confocal

SCI ; EI

英语

NI期刊 ; NI论文

通讯

Shenzhen Peacock Program Grant[KQTD20140630160825828]

Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics

Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter

WOS:000428793600012

WILEY-V C H VERLAG GMBH

20180804810417

Biomolecules ; Fluorescence imaging ; Fluorescence microscopy ; Fluorophores ; Infrared devices ; Tissue

Biological Materials and Tissue Engineering:461.2 ; Biology:461.9 ; Light/Optics:741.1 ; Imaging Techniques:746 ; Quantum Theory; Quantum Mechanics:931.4

MATERIALS SCIENCE

Web of Science

1.Stanford Univ, Dept Chem, Stanford, CA 94305 USA
2.Stanford Univ, Sch Med, Dept Obstet & Gynecol, Program Reprod & Stem Cell Biol, Stanford, CA 94305 USA
3.IVI Fdn, Valencia 46026, Spain
4.South Univ Sci & Technol China, Dept Mat Sci & Engn, Shenzhen 518055, Peoples R China
5.Tsinghua Univ Shenzhen, Res Ctr Adv Mat & Biotechnol, Res Inst, Shenzhen 518057, Peoples R China

Zhu, Shoujun,Herraiz, Sonia,Yue, Jingying,et al. 3D NIR-II Molecular Imaging Distinguishes Targeted Organs with High-Performance NIR-II Bioconjugates[J]. ADVANCED MATERIALS,2018,30(13).

Zhu, Shoujun.,Herraiz, Sonia.,Yue, Jingying.,Zhang, Mingxi.,Wan, Hao.,...&Dai, Hongjie.(2018).3D NIR-II Molecular Imaging Distinguishes Targeted Organs with High-Performance NIR-II Bioconjugates.ADVANCED MATERIALS,30(13).

Zhu, Shoujun,et al."3D NIR-II Molecular Imaging Distinguishes Targeted Organs with High-Performance NIR-II Bioconjugates".ADVANCED MATERIALS 30.13(2018).