Ferrostatin-1 alleviates lipopolysaccharide-induced acute lung injury via inhibiting ferroptosis

Liu, Pengfei1,2; Feng, Yetong3; Li, Hanwei1,4; Chen, Xin5; Wang, Guangsuo6; Xu, Shiyuan4; Li, Yalan2,7; Zhao, Lei1,2

2020-02-27

10.1186/s11658-020-00205-0

CELLULAR & MOLECULAR BIOLOGY LETTERS   影响因子和分区

1425-8153

1689-1392

Background Ferroptosis is a newly recognized type of cell death, which is different from traditional necrosis, apoptosis or autophagic cell death. However, the position of ferroptosis in lipopolysaccharide (LPS)-induced acute lung injury (ALI) has not been explored intensively so far. In this study, we mainly analyzed the relationship between ferroptosis and LPS-induced ALI. Methods In this study, a human bronchial epithelial cell line, BEAS-2B, was treated with LPS and ferrostatin-1 (Fer-1, ferroptosis inhibitor). The cell viability was measured using CCK-8. Additionally, the levels of malondialdehyde (MDA), 4-hydroxynonenal (4-HNE), and iron, as well as the protein level of SLC7A11 and GPX4, were measured in different groups. To further confirm the in vitro results, an ALI model was induced by LPS in mice, and the therapeutic action of Fer-1 and ferroptosis level in lung tissues were evaluated. Results The cell viability of BEAS-2B was down-regulated by LPS treatment, together with the ferroptosis markers SLC7A11 and GPX4, while the levels of MDA, 4-HNE and total iron were increased by LPS treatment in a dose-dependent manner, which could be rescued by Fer-1. The results of the in vivo experiment also indicated that Fer-1 exerted therapeutic action against LPS-induced ALI, and down-regulated the ferroptosis level in lung tissues. Conclusions Our study indicated that ferroptosis has an important role in the progression of LPS-induced ALI, and ferroptosis may become a novel target in the treatment of ALI patients.

Ferrostatin-1 Ferroptosis Lipopolysaccharide Acute lung injury

SCI

英语

ESI高被引

第一 ; 通讯

Science and Technology Planning Project of Guangdong Province[2016ZC0244]

Biochemistry & Molecular Biology ; Cell Biology

Biochemistry & Molecular Biology ; Cell Biology

WOS:000519921100001

BMC

MOLECULAR BIOLOGY & GENETICS

Web of Science

1.Southern Univ Sci & Technol, Jinan Univ, Shenzhen Peoples Hosp, Dept Anesthesiol,Clin Med Coll 2,Affiliated Hosp, Shenzhen 518020, Peoples R China
2.Jinan Univ, Integrated Chinese & Western Med Postdoctoral Res, Guangzhou 510632, Peoples R China
3.Shenzhen Univ, Hlth Sci Ctr, Sch Basic Med Sci, Shenzhen 518037, Peoples R China
4.Southern Med Univ, Dept Anesthesiol, Zhujiang Hosp, Guangzhou 510280, Peoples R China
5.Southern Univ Sci & Technol, Affiliated Hosp 1, Dept Lab Med, Clin Med Coll 2,Shenzhen Peoples Hosp,Jinan Univ, Shenzhen 518020, Peoples R China
6.Southern Univ Sci & Technol, Affiliated Hosp 1, Shenzhen Peoples Hosp, Dept Thorac Surg,Clin Med Coll 2,Jinan Univ, Shenzhen 518020, Peoples R China
7.Jinan Univ, Dept Anesthesiol, Affiliated Hosp 1, Guangzhou 510632, Peoples R China

Liu, Pengfei,Feng, Yetong,Li, Hanwei,et al. Ferrostatin-1 alleviates lipopolysaccharide-induced acute lung injury via inhibiting ferroptosis[J]. CELLULAR & MOLECULAR BIOLOGY LETTERS,2020,25(1).

Liu, Pengfei.,Feng, Yetong.,Li, Hanwei.,Chen, Xin.,Wang, Guangsuo.,...&Zhao, Lei.(2020).Ferrostatin-1 alleviates lipopolysaccharide-induced acute lung injury via inhibiting ferroptosis.CELLULAR & MOLECULAR BIOLOGY LETTERS,25(1).

Liu, Pengfei,et al."Ferrostatin-1 alleviates lipopolysaccharide-induced acute lung injury via inhibiting ferroptosis".CELLULAR & MOLECULAR BIOLOGY LETTERS 25.1(2020).